Information-enriched characterization of mobile populations. Morphological data from illustrations or photos can incorporate towards the richness of flow cytometry info. Such as, the figure shows an in any other case standard apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Every population to expose morphologically distinct options. These insights could not are actually gained from multiplex staining on your own.
As samples are acquired within the Attune CytPix Flow Cytometer, the significant-velocity brightfield digital camera captures and outlets illustrations or photos of detected activities in a rate up to six,000 images for each next, depending upon the flow rate and impression sizing. For bigger versatility, Attune Cytometric Software program permits you to adjust the impression capture frequency as wanted and choose a selected gate for picture capture, if expected.
Mix acoustic concentrating technological innovation with higher-parameter analysis for adaptable spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing by using a significant-velocity brightfield digital camera for simultaneous higher throughput flow cytometry and high resolution brightfield imaging.
"A picture isn't rather truly worth one thousand dots, nonetheless it genuinely really helps to see illustrations or photos. It is excellent to see photos that correlate with staining. For example, AnnexinV-optimistic cells which are morphologically different than balanced cells, or CD14+ monocytes which might be bigger and even more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it really is illuminating to see what number of doublets slip by way of common singlet gates, and excellent to validate the position of sudden double-beneficial events in my Assessment."
Optimizing gating technique. Even strong handbook singlet gating is error-susceptible and remains a subjective final decision place in Just about all flow cytometry assays. Imaging can be employed to substantiate and change gates to include only single cells of interest.
Constant image top quality even at substantial throughput Acoustic focusing as well as a superior-pace digicam Incorporate to graphic these T cells consistently at minimal or significant flow costs. Very easily regulate aim and camera configurations to fulfill experimental demands.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can depend upon for reliable and trustworthy effectiveness. The Attune CytPix design encompasses a substantial-pace brightfield camera and AI-driven impression analysis program, with recent upgrades such as machine Discovering equipment and an intuitive user interface, enabling enhanced details precision, precision, and quicker insights into sample biology.
People can watch function visuals in authentic-time to verify sample good quality, enhance protocols, and file the morphology of each cell populace identified for Assessment. Imaging seize also Gains from acoustic concentrating, which helps to place the cells so that a pointy, centered impression is received.
Seem waves permit precise mobile alignment, leading to Outstanding information high quality and ~10X more quickly sample flow costs.
Application note: Automated image Investigation decreases user-to-user variability in flow cytometry gating methods
Leverage the strength of advanced technology and innovation to accelerate your discoveries. Belief from the Attune Flow Cytometer family to provide the trustworthiness, velocity, and precision that set your function aside.
No wash/no lyse leukocyte Examination with imaging. Cells had been acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Specialist UX/UI permits buyers to practice AI versions for expanded programs. (A) The “Prepare Product” characteristic gives annotation instruments and authentic-time design teaching precision.
Gating on these extended parameters lets you rapidly and accurately detect populations of interest and ensure gating technique with little if any manual evaluate. The Attune software delivers access to state-of-the-artwork device Studying Investigation equipment in just an expertly designed consumer interface, rendering it extremely easy to use.
The Attune Xenith cytometer is usually a significant-parameter spectral here analyzer, offering both equally spectral unmixing and traditional flow abilities for in depth and flexible cell analysis.
Agilent Price Promise guarantees you at the very least 10 years of instrument use from your date you buy. In any other case, We are going to credit history you With all the residual worth of the system toward an upgraded design.
What appeared to be a homogeneous population is definitely more various—an Perception that needs to be thought of when interpreting results.